Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, cytotoxicity and genotoxicity investigations

Cryogelation has become an advantageous method to obtain macro-porous materials with well-defined, interconnected pores for tissue engineering applications. Herein, polyvinyl alcohol and chitosan polymers (PVA-CHI) were used produce cryogel scaffolds via cryogelation. Glutaraldehyde was as a crosslinking agent the effect of amount on properties investigated. divided into 5, 10, 15% total polymer concentration. The optimized pore morphology obtained scaffold containing 5% glutaraldehyde amount. In addition FTIR, SEM, swelling, degradation analyses, mechanical tests performed present characterization cryogels. Direct indirect cytotoxicity test genotoxicity experiments Mouse Embryonic Fibroblasts (MEF). addition, cell morphologies analyzed SEM. results showed that PVA-CHI based cryogels had no genotoxic cytotoxic effects MEF cells have potential